The encounter ended in a 1-1 stalemate and Matsatsantsa went on to win 4-2 penalties with goalkeeper Ronwen Williams saving two spot-kicks.
This is the second time the Tshwane giants have won this tournament having clinched it in 2004 when it was still known as the SAA Supa 8 Cup.
Here is how Twitter reacted to SuperSport's victory over Benni McCarthy's City:
Congratulations to Supersport United for winning the MTN8 final by 4 goals to 2 in a game that went into extra time.— Marks Maponyane (@MarksMaponyane) October 14, 2017
Penalties are the worst nightmare in football. Hard Lucky Benni McCarthy, Congratulations to SuperSport United. #MTN8Final— IG @AdvBarryRoux (@AdvBarryRoux) October 14, 2017