The England international has overcome an ankle injury but will not feature from the start against the Blues.
Instead, Heung-Min Son will lead the line for Spurs, having scored 18 goals thus far this season in all competitions, including 12 in the Premier League.
He will be supported by Erik Lamela, Dele Alli and Christian Eriksen, with January signing Lucas Moura, acquired from PSG, on the bench.
Morata has not played 90 minutes in the Premier League since the club's 2-1 loss to Manchester United in February and has yet to score a league goal this year, last finding the net against Brighton on Boxing Day.
Elsewhere, Willy Caballero plays in goal after Thibaut Courtois failed to overcome a hamstring injury in time for the derby encounter. The Belgian is not fit enough for the bench, and is left out of the squad altogether.
Andreas Christensen, however, returns to the fold after leaving international duty with Denmark early due to a muscular injury.
The clash has added importance for the Blues, who are aware that they could slip eight points behind Spurs if Mauricio Pochettino's side are able to win at Stamford Bridge for the first time in the Premier League era.
Chelsea team: Caballero; Azpilicueta, Christensen, Rudiger; Moses, Kante, Fabregas, Alonso; Willian, Hazard; Morata. Subs: Eduardo, Cahill, Emerson, Drinkwater, Bakayoko, Hudson-Odoi, Giroud.
Spurs team: Lloris; Trippier, Sanchez, Vertonghen, Davies; Dier, Dembele; Lamela, Alli, Eriksen; Son. Subs: Vorm, Aurier, Rose, Wanyama, Sissoko, Lucas, Kane.