Dembele, 21, came off early in the second half at Camp Nou after picking up an injury following a tackle from Raul Navas.
"The first team player Ousmane Dembele has a sprained right ankle," a club statement read.
"In the next few days he will continue under the supervision of the club's medical staff."
The injury is a slight worry for France less than a month out from the World Cup in Russia.
Dembele is part of Didier Deschamps' squad, who begin their tournament with a clash against Australia on June 16.