That loss put an end to an unbeaten streak that had stretched to 19 games and they will be eager to bounce back by returning to winning ways as they seek to move closer to Primera Division glory.
Valencia, meanwhile, are on a nine-game unbeaten run - eight of which were victories - and they can move level with second-place Atletico Madrid with a win in Catalunya.
|Game||Barcelona vs Valencia|
|Date||Saturday, April 14|
|Time||15:15 BST / 10:15 ET|
|Stream (US only)||fubo TV (7-day free trial)|
TV Channel & Live Stream
In the United States (US), the game can be watched live and on-demand with fuboTV (7-day free trial).
New users can sign up for a free seven-day trial of the live sports streaming service, which can be accessed via iOS, Android, Chromecast, Amazon Fire TV, Roku and Apple TV as well as on a web browser.
|US TV channel||Online stream|
|beIN Sports||fubo TV (7-day free trial)|
In the United Kingdom (UK), the game cannot be watched live on TV or streamed live online, but it can be followed live with Goal by clicking here.
|UK TV channel||Online stream|
Squads & Team News
|Goalkeepers||Ter Stegen, Cillessen, Ortola|
|Defenders||Semedo, Pique, Alba, Umtiti, Vermaelen|
|Midfielders||Busquets, D. Suarez, Iniesta, Coutinho, Paulinho, Roberto, Gomes|
|Forwards||L. Suarez, Messi, Dembele, Alcacer|
Barca will be able to call upon Philippe Coutinho, who was cup-tied for the Champions League and Sergio Busquets has been named in the 18-man panel for the game.
Ivan Rakitic will not take part after it emerged that he would undergo surgery on a fractured finger.
Potential Barcelona starting XI: Ter Stegen; Semedo, Pique, Umtiti, Alba; Paulinho, Roberto, Coutinho, Dembele; Messi, L. Suarez.
|Goalkeepers||Domenech, Neto, Rivero|
|Defenders||Vezo, Murillo, Gabriel, Gaya, Lato, Montoya, Garay, Jiminez, Vidal|
|Midfielders||Maksimovic, Guedes, Parejo, Pereira, Kondogbia, Coquelin, Soler|
|Forwards||Vietto, Zaza, Rodrigo, Torres, Mina|
Valencia will be without Toni Lato, who has a hamstring problem, while Francis Coquelin is out due to an Achilles issue. Javi Jiminez is also out of action with a knee injury.
Potential Valencia starting XI: Neto; Garay, Gabriel, Montoya, Gaya; Kondogbia, Parejo, Guedes, Soler; Zaza, Rodrigo.
Betting & Match Odds
Barcelona are heavy favourites to win at odds of 3/10, while Valencia are rated with a 17/2 chance of beating the Blaugrana. A draw is available at 9/2.
While they crashed out of the Champions League in spectacular fashion on Tuesday, Barcelona remain on course for a double, with league and Copa del Rey honours still within their grasp.
Incredibly, the Blaugrana are unbeaten in the league after 31 games - winning 24 - and they are 11 points clear of their nearest rivals Atletico coming into the game against Valencia.
However, Marcelino Garcia Toral's men are one of the few teams who have been able to deny Barca a win this season, having drawn 1-1 at Mestalla back in November last year.
Striker Rodrigo put Los Che ahead in that match and Barca needed a late intervention from Jordi Alba to rescue a point, so Saturday's game is primed to be a tight affair.
Indeed, Valencia are without doubt the form team in La Liga ahead of the game and Valverde's men will have to be vigilant after their loss to Roma if they are to maintain their unbeaten record.