Ernesto Valverde's side went down 3-0 at the Stadio Olimpico on Tuesday to crash out of Europe on away goals after a 4-4 aggregate result.
The Blaugrana are still unbeaten in the Primera Division, however, and will look to extend their record this season to 32 games as they close in on the title, with second-placed Atletico 11 points back.
Here, Goal takes a look at everything you need to know ahead of Saturday's game at Camp Nou...
Ivan Rakitic has been left out after picking up a knock against Roma on Tuesday. Meanwhile, Sergi Samper is out with a long-term injury and is unlikely to feature for the Catalan club this season. Lucas Digne is doubtful.
Valverde has no players suspended for the game against Valencia.
Barcelona potential starting line-up
Philippe Coutinho, who was cup-tied for the Champions League after turning out for Liverpool in the competition earlier this season, is set to return to the side on Saturday.
Elsewhere, Sergio Busquets (who wore a reinforced boot on Tuesday following a toe problem and may do so again this weekend) is set to partner Paulinho in midfield as Rakitic sits out.
Valverde must decide between Sergi Roberto and Nelson Semedo for the right-back position, but the coach will pick his strongest side for the visit of third-placed Valencia.
Valencia team news
Marcelino Garcia Toral's men have enjoyed a spectacular season and are virtually guaranteed a return to the Champions League. Having won their last five league games, they are one point above Real Madrid and three behind Atletico, although they are 14 adrift of Barcelona.
Defender Javi Jimenez and midfielder Francis Coquelin are sidelined and will play no part on Saturday.
TV channel & kick-off time
Barcelona versus Valencia kicks off at 16:15 local time on Saturday and as games cannot be broadcast live in the UK (United Kingdom) at that time, highlights will be shown later on Sky Sports Football at 23:30 BST.
In the United States, the game can be watched live on television on beIN SPORTS and kicks off at 10:15 ET.
Best Opta match facts
Barcelona are unbeaten in their last 38 La Liga games (won 31, drawn seven) and they could surpass Real Sociedad in 1979-80 to set a new record of consecutive unbeaten games for any team (39).
Valencia have won two of their last four league visits to Camp Nou (losing the other two), scoring seven goals in the four games.
Lionel Messi has scored 29 goals in La Liga this season and he could be the first ever player to reach 30+ goals in seven different campaigns.
Luis Suarez has netted nine goals against Valencia in all competitions, his joint-favourite opponent with Barcelona, alongside Betis.
Rodrigo Moreno has won more points with his goals than any other player in La Liga this season (16 points with 15 goals).