Barcelona were hammered 4-0 in the first-leg of the last-16 clash at Parc des Princes, the result equaling their heaviest-ever defeat suffered in Europe.
Luis Enrique's men face tall odds setting out to turn around the tie and join Bayern Munich and Real Madrid in the quarter-finals, with the coach lining up his men in an unconventional, attacking 3-3-1-3 formation.
No side has ever gone through after losing a first-leg 4-0, with the Spanish champions eager to ensure they reach the last eight for the 10th season in a row.
Messi has been deployed in a free role behind Rafinha, Suarez and Neymar as they look to score five goals or more in a bid to wrest back control of the tie in front of their own fans.
Key will be Barcelona's ability to prevent PSG netting a crucial away goal, with Gerard Pique, Javier Mascherano and Samuel Umtiti charged with stopping PSG hitman Edinson Cavani.
The Uruguayan has netted seven times in the competition thus far - his best tally ever in the Champions League - and will be hoping to fire Les Parisiens to the quarter-finals for the fifth campaign in a row.
Revitalised Julian Draxler is operating in support of Cavani, while Di Maria - PSG's hero of the first-leg - has been surprisingly left on the bench as former Sevilla boss Unai Emery opts for a more solid option in Blaise Matuidi in his place.
Barcelona XI: Ter Stegen; Pique, Mascherano, Umtiti; Rakitic, Busquets, Iniesta; Messi; Rafinha, Suarez, Neymar.
Subs: Arda Turan, Alcacer, Digne, Andre Gomes, Cillessen, Alba, Sergi Roberto.
PSG XI: Trapp; Meunier, Marquinhos, Thiago Silva, Kurzawa; Rabiot, Verratti, Matuidi; Lucas Moura, Cavani, Draxler.
Subs: Kimpembe, Pastore, Areola, Ben Arfa, Krychowiak, Di Maria, Aurier.