Mbappe clashed heads with Houssem Aouar before being carried off on a stretcher following a heavy collision with Lyon goalkeeper Anthony Lopes prior to half-time.
The 19-year-old underwent extensive tests and visited a neurologist, with head coach Unai Emery stating on Tuesday that the attacker wanted to take part in the Coupe de France clash at Stade de Roudourou.
However, despite avoiding a serious injury, Mbappe will not be risked against Guingamp, a match Neymar will also miss with a thigh strain.
"After 48 hours, [Mbappe] is still suffering from bruising. However, additional tests have found no evidence of traumatic injuries," PSG tweeted.
"[Neymar] did not train today [Tuesday]. He remains sidelined with a right thigh injury."
Mbappe has scored 15 goals in all competitions in 2017-18, his first season with PSG after a summer move from Monaco.