Madrid's 2-1 defeat at Valencia on Wednesday means Zinedine Zidane's side still lead La Liga by a single point, while they also have a game in hand, but a win for Luis Enrique's men at the Vicente Calderon would see the Blaugrana move provisionally into top spot - with Real in action at Villarreal later on Sunday.
Aleix Vidal is sidelined for the remainder of the season after suffering a horrific ankle injury in the recent win at Alaves. The former Sevilla right-back is likely to be out for five months and Barca are allowed to sign a replacement if they so decide.
Javier Mascherano is a doubt for the game against Diego Simeone's side after a spell out, but Arda Turan could return after missing the last two weeks through injury.
Sergio Busquets was suspended for the game against Leganes last weekend, but the midfielder returns on Sunday and Luis Enrique has no other players banned for the trip to the Vicente Calderon.
BARCELONA POTENTIAL STARTING LINE-UP
Luis Enrique is set to name a strong side for the trip to Atletico Madrid in La Liga on Sunday - the last time Barcelona will play a league game at the Vicente Calderon before the stadium is knocked down later this year.
With Javier Mascherano doubtful and Aleix Vidal sidelined, there will be few susprises in the defence as Sergi Roberto, Jordi Alba, Gerard Pique and Samuel Umtiti are all likely to start.
In midfield, Arda Turan could return against his former club, but will probably have to settle for a place on the bench, with either Ivan Rakitic or Andre Gomes alongside Sergio Busquets (back from suspension) and Andres Iniesta.
Lionel Messi, Luis Suarez and Neymar will be together in attack as well, while Luis Enrique could use this fixture to try out the 4-2-3-1 formation he is looking at ahead of the return against Paris Saint-Germain next month.
ATLETICO TEAM NEWS
Atletico secured an excellent win in the Champions League on Tuesday as they beat Bayer Leverkusen 4-2 in Germany to take command of the tie ahead of the return match at the Vicente Calderon next month.
Diego Simeone's side are currently fourth in La Liga, four points behind third-placed Sevilla, and will hope to seal a victory in this match to move closer to the top three and automatic qualification for next season's Champions League.
Tiago, Juanfran and Augusto Fernandez are all out through injury and will not feature against Barca, but Diego Godin could play a part after returning to training on Friday.
BEST OPTA MATCH FACTS
Atletico Madrid have won their last three Liga games, but Diego Simeone's side are yet to win four in a row in the Primera Division this season.
Barcelona are one of two Liga teams unbeaten against Diego Simeone (drawn three, lost 10 against the Blaugrana), alongside Alaves (drawn two).
This will be Diego Simeone’s 300th game in all competitions as Atletico coach.
Only against Sevilla (27) has Lionel Messi scored more goals than against Atletico (26) in all competitions.
TV COVERAGE & KICK-OFF TIME
Atletico versus Barca kicks off at 16:15 CET on Sunday afternoon and will be shown live in the UK on Sky Sports 2 from 14:45 GMT.