Ole Gunnar Solskjaer's team suffered a blow to their top four aspirations after going down 2-0 at the Emirates Stadium, his first league defeat since taking charge in November.
Having overseen victories over Arsenal and Chelsea in the last two rounds, the interim boss is priced at 13/10 (2.30) with bet365 to secure a place in the semi-finals with another impressive win here.
This pair shared a 1-1 draw at Old Trafford when they met in the league back in September, and there are odds of 23/10 (3.30) available on this game heading into extra-time after another deadlocked 90 minutes.
The hosts have a fully fit squad to choose from, with Ryan Bennett available to play despite his two-match ban in the Premier League.
Ander Herrera and Phil Jones could be in contention after returning from injury and illness respectively, although Juan Mata, Jesse Lingard, Alexis Sanchez, Matteo Darmian, and Antonio Valencia remain sidelined.
Following on from their thrilling comeback Champions League victory over PSG, the defeat in north London was a disappointing comedown for the Red Devils.
They could be in for another frustrating 90 minutes against Nuno Espirito Santo's men, who put in an extremely conservative performance in holding Chelsea to a 1-1 draw at Stamford Bridge last weekend.
Indeed, Wolves have conceded only four goals in their last six matches, while scoring just six times at the other end of the pitch.
With United having let in just six goals in 10 away games under Solskjaer, odds of 4/5 (1.80) look like solid value on under 2.5 being scored for their second meeting in succession with this opposition.
All odds correct at time of publish. Please gamble responsibly.