Dave Sarachan saw his side beat their neighbours 1-0 in Tennessee and will be hoping for more success against a Colombia side playing their third consecutive friendly on American soil.
These two sides have met 19 times previously dating back to 1961, with Colombia winning 12 of those encounters.
The South Americans have also won each of the last three meetings, with the most recent coming in the Copa America in 2016, and bet365 offer another Colombian win at 17/20 (1.85). The USMNT can be backed at 10/3 (4.33) with the draw available at 12/5 (3.40).
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Midfielder Tyler Adams scored the winner for the US against Mexico but is missing through injury along with Christian Pulisic and West McKennie.
Colombia's interim coach Arturo Reyes has named four uncapped players in the squad, along with the side's most important players in Radamel Falcao, James Rodriguez and Juan Cuadrado.
Each of Colombia's last two victories against the US have come without conceding.
In fact, four of the last five meetings between these two sides have seen Los Cafeteros keep a clean sheet and the South Americans come into the clash having also done so against Argentina.
The US are missing some key players and may struggle once more to find the back of the net as they did the last time they faced CONMEBOL opponents, a 2-0 loss to Brazil in September.
Tips and predictions
Colombia are priced at 5/4 (2.25) to keep a clean sheet and given their record against the US, this looks like a solid option for the clash in Florida.
Prices correct at the time of writing. Please gamble responsibly.