Steven Gerrard's side had gone 11 matches without defeat in European competition this term before being beaten 4-3 by Spartak Moscow in the Russian capital earlier this month.
It was a particularly painful defeat for the Gers, for they had led on no fewer than three occasions and were denied a point in stoppage time by the woodwork.
There is no room for the Scots to feel sorry for themselves, though, for just two points separate the four teams in Group G and they could go top with victory. SportPesa offer 47/20 (3.35) that the hosts deliver in Govan.
Villarreal lead the way with six points and will be assured of a place in the last 32 should they win in Govan - an outcome that is chalked up as a 21/20 (2.05) chance with SportPesa.
These teams played out a 2-2 draw in the group opener on Spanish soil in September and SportPesa go 5/2 (3.50) that this contest also ends all square.
Jamie Murphy, Graham Dorrans and Ryan Kent are out for the hosts, while Borna Borisic is a doubt. Alfredo Morelos scored twice in Rangers' 3-1 home win against Rapid Vienna and Gerrard will hope the Colombian striker is again in fine form ahead of the La Liga side's visit.
Villarreal will have to do without the services of Morelos' fellow countrymen, Carlos Bacca, who is sidelined through injury. Bacca scored when the sides met in September.
Villarreal have failed to win any of their last six matches away from home, but the La Liga side have scored in seven of their last eight matches on their travels.
SportPesa go 20/23 (1.85) that Javi Calleja's men score more than one goal in Govan, while the same firm go 5/7 (1.71) that there are more than two goals in the game - the selection would have landed in all but one of Villarreal's last seven away games.
Rangers have scored 30 goals in their last nine home games and, so, there is plenty of potential for the goals to flow here. Indeed, SportPesa offer 5/8 (1.63) that there are goals at both ends.
Better value, though, may lie in backing at least three goals to be scored - and both teams to score - in Glasgow at 20/21 (1.95) with SportPesa.