Title holders Bechem United, after eliminating Real Apostles, have been handed second-tier side Guan United while Asante Kotoko and Hearts of Oak will lock horns with Proud United and Kotoku Royals respectively.
Wafa have been paired with Unistar Academy while Bolga All Stars, who eliminated Real Tamale United will travel to Wa to face Ghana Premier League title holders Wa All Stars.
There will also be a Brong Ahafo derby when Berekum Chelsea host B.A United at the Golden City Park.
The matches are expected to take place between May 18 and 22 May.
Hearts of Oak vs Kotoku Royals
Unistar Academy vs WAFA
Proud United vs Asante Kotoko
Nea Salamina vs Techiman XI Wonders
Wa All Stars vs Bolga All Stars
Berekum Chelsea vs BA United
Bechem United vs Guan United
Steadfast FC vs Power SC
King Faisal FC vs Wassaman United
Gold Stars vs Elmina Sharks
Samatex 1996 FC vs Asokwa Deportivo
Medeama SC vs New Edubiase FC
Liberty Professionals vs Uncle T Stars
Danbort FC vs Heart of Lions
Young Wise vs Okyeman Planners
Emmanuel FC vs Amidaus Professionals