Brazil international Fernandinho was due to undergo a scan on Thursday after limping out of the 2-0 derby win over Manchester United in midweek, which lifted Guardiola's men back to the top of the table – a point ahead of title rivals Liverpool with three games to play.
City have not released the results of the injury assessment and will instead give the 33-year-old time to prove his fitness before the trip to Turf Moor on Sunday.
Fernandinho's possible inclusion in the travelling party would serve as a major boost to the champions, who will again be without Kevin De Bruyne due to a hamstring issue.
"We will see tomorrow," Guardiola said of Fernandinho's availability at Friday's pre-match news conference.
"He has a problem with his knee, but we will see tomorrow [whether he can play].
"[De Bruyne is] still not fit. He's getting better, his clinical feeling is better, but he's still not ready."
Another great result! Keep pushing. We are city +3 pic.twitter.com/bPg96ZJEMm— Fernandinho (@fernandinho) April 24, 2019
There are no such fitness concerns over centre-back John Stones despite his absence from the squad that took a potentially huge step towards the title at Old Trafford.
"It was a decision based on what I saw," Guardiola said.
"Nico has been incredible in the last few months. I try to involve everybody but for certain games, we need specific qualities in defence and that is why I went with Vinny and Nico.
"Vinny is a little bit quicker and has the personality to play at Old Trafford. He has played in a lot of derbies.
"It also depends on what I see in the training sessions - who is happy, who is down - but not for the quality of the players [is anyone being left out]."