The star forward entered the contest needing just a single tally to break a 23-year-old record that had been equaled but never bested.
The first goal of the contest did not fall to Martinez, as his team-mate Leandro Gonzalez Pirez gave Atlanta an early lead with a goal in the 21st minute.
Orlando City would equalizer later in the first half behind Scott Sutter, setting the stage for Martinez to make history.
Martinez broke into the Orlando City area after being played in by Julian Gressel in the 74th minute. The forward had a nice touch to take him past Jonathan Spector and flicked it past Lions goalkeeper Joe Bendik to not only re-establish his side's lead, but also put his name in the history books.
The goal proved the difference as the Five Stripes walked away 2-1 winners to build on their league-best record.
Lassiter set the initial mark in 1996, the league's initial season, while playing for the Tampa Bay Mutiny.
2️⃣8️⃣ pic.twitter.com/8rZx3GFYpH— Major League Soccer (@MLS) August 25, 2018
Incredibly, Martinez has managed the feat in just 26 games, with Lassiter previously the best, having scored 27 in 30 games. Wondolowski and Wright-Phillips finished on 27 goals after playing in 32 games in their season.
While an injury limited his debut season in MLS, Martinez still managed to bag 19 goals in 20 games for the then-expansion MLS side.
His 28 in 26 games so far this season, Martinez now has scored an incredible 47 goals in 46 games in the MLS regular season.
Martinez will get a chance to extend his record when Atlanta United next play on September 3 against D.C. United.