Tunisian champions Esperance opened their group stage campaign with a 0-0 draw with Al Ahly away last month.
They wil now host Rollers at the Stade Olympique de Radès in the Tunisian Harbour city of Radès.
Third-placed Esperance are looking for a victory which would take them to the top of the group standings if other results go their away.
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Esperance's key striker Taha Yassine Khenissi will be looking to rediscover his scoring form against Rollers and help his side win at home.
They will face Rollers side oozing with confidence having marked its debut in the group stage with a 1-0 home win over KCCA.
As a result, the Botswana champions are sitting at the top of the Group A standings with three points from one game.
Rollers wil be hoping to cause an upset against Esperance and remain at the top of the group table.
Lemponye Tshireletso, who scored against KCCA, is Rollers' dangerman and he could be key to Rollers' win over Esperance.
Match referee Bamlak Tessema Weyesa of Ethiopia will be in charge of the game between Esperance and Rollers.
Meanwhile, KCCA, whose full name is Kampala Capital City Authority, will look to bounce back from their defeat to Rollers.
The defeat left the Ugandan champions, who are also making their debut in the group stage, placed fourth on the Group A standings.
They have a chance to redeem themselves when they host Al Ahly at the Mandela National Stadium in Uganda's second largest city, Kira.
Promising forward Muhammad Shaban, who has been one of KCCA's key attackers this season, will look to score and help his side stun Al Ahly.
They will face an Al Ahly side keen to record its first win in Group A after being frustrated by Esperance at home.
Record eight-time Champions League winners Al Ahly are heading into this encounter placed second on the group standings.
The Egyptian football heavyweights will look to Mido Gaber, who is a key attacker for the Red Devils.
South African match referee Victor Gomes will be in charge of the game between KCCA and Al Ahly.