The Egyptians released the news on Monday, stating that the Nigeria has been included to face the Algerians.
Al Ahly have been without Ajayi for five months after he suffered a knee ligament injury against Township Rollers on July 28.
But the 22-year-old is now available to return, as his side chase a vital away victory to Algeria.
He returns to Martín Lasarte's squad alongside Ahmed Fathi, while Saad Samir was excused from the clash.
Ahly began their Group D campaign with a 2-0 victory over AS Vita Club at the Borg El Arab Stadium, Alexandria.
Goalkeepers: Sherif Ekramy, Mohamed El-Shennawy, Ali Lotfi.
Defenders: Ayman Ashraf, Ali Maaloul, Mohamed Hany, Yasser Ibrahim.
Midfielders: Ahmed Fathi, Karim Nedved, Mohamed Sherif, Ahmed Hamoudi, Islam Mohareb, Amr El-Sulaya, Ramadan Sobhi, Nasser Maher, Walid Soliman, Mido Gaber.
Forwards: Geraldo, Junior Ajayi.