VAR technology was introduced this season in Italy's top flight and the Sardinians were the first beneficiaries of a reversed decision on the first day of the Serie A season – though Gianluigi Buffon saved from Diego Farias' poor spot-kick.
Near the end of the first half, Duje Cop went down under a challenge from Juventus defender Alex Sandro, but the referee only awarded a corner.
But the referee was notifiied that the VAR determined the incident could have been a penalty kick and after a brief review, a spot-kick was awarded to Cagliari.
Farias was unable to take advantage, however, as his poor penalty was easily saved by Buffon.
Juventus would go on to win the opener 3-0 after goals from Mario Mandzukic, Paulo Dybala and Gonzalo Higuain.