Van Dijk, 26, is heading to Anfield from Southampton in January after the clubs agreed a deal reportedly worth £75 million.
He underwent a medical with Liverpool on Wednesday and, as well as becoming the most expensive defender in history, the move will represent the highest fee ever paid by the Reds
However, speaking after City's 1-0 win over Newcastle United, Guardiola was asked if Van Dijk was a player he was looking at in January.
"Nope. No, no, no," the Spaniard responded.
Asked if he was interested in Van Dijk, Guardiola added: "No."
City are 15 points clear at the top of the Premier League and have won 19 of their 20 games in the competition, though the victory over Newcastle was soured by an injury to captain Vincent Kompany.