Messi was absent from Barca's starting XI for the visit of Leganes in La Liga on Sunday, coming on as a substitute to spare their blushes – playing a role in Luis Suarez's goal before also scoring himself.
Coach Ernesto Valverde acknowledged in the lead up to that game that he was likely to take opportunities to rest Messi in the coming weeks ahead of a busy period, which includes the return of the Champions League.
Sevilla – a team he boasts a fine record against – are the lucky beneficiaries of his time out of the side, as Barca travel to Andalusia for the first leg of their Copa quarter-final.
However. Boateng is in line to make his first appearance for the club.
The 31-year-old makes his return to Spanish football having previously featured for Las Palmas.
Young talent Riqui Puig is included in the squad as well, with opponents Sevilla aiming to arrest a run of three successive defeats in all competitions.