The Argentine maestro missed both of his country's friendlies in the most recent international break due to a hamstring injury.
Elsewhere, Sergio Busquets is also fit for the Blaugrana after missing three weeks due to suffering a broken bone in his foot against Chelsea on March 14.
Ousmane Dembele must be content with a place on the bench, as Sergi Roberto has been given a start in midfield.
For Roma, Cengiz Under and Radja Nainggolan are unavailable due to injury, while Lorenzo Pellegrini has passed a late fitness test.
Barcelona XI: Ter Stegen; Semedo, Pique, Umtiti, Alba; Iniesta, Busquets, Rakitic, Roberto; Messi, Suarez.
Subs: Cillessen, Denis Suarez, Dembele, Paulinho, Alcacer, Andre Gomes, Vermaelen
Roma XI: Alisson; Peres, Manolas, Fazio, Kolarov; Pellegrini, De Rossi, Strootman; Florenzi, Dzeko, Perotti
Subs: Skorupski, J. Jesus, Gonalons, Gerson, El Shaarawy, Schick, Defrel