Playing at home, top-flight Hearts could not make the most of their advantage as they could only settle for a 1-1 draw with lower division club UniStar Academy. Kojo Obeng Junior gave the Phobians a first-half lead but Joshua Jessee levelled the score in injury time for UniStar who went on to register a famous 5-4 win on penalties.
City rivals and fellow Premier League side Liberty were not lucky in their home fixture against Division One outfit Proud United who similarly claimed a 4-2 win on penalties following a goalless draw in regulation time. Proud United and UniStar will face off in an all-lower division showdown in the quarter-finals.
Premier League giants Asante Kotoko, on the other hand, proved their supremacy with a 2-1 victory over Division One club Nkoranza Warriors. Augustine Sefah's late strike was the difference as Warriors cancelled Daniel Nii Adjei's opener for the Porcupines. Kotoko captain Amos Frimpong missed a penalty in the game.
In the next stage, Kotoko will face Berekum Arsenals who pipped BA United 3-2.
A 2-1 victory over New Edubiase United has confirmed Ashanti Gold among the teams for the next round where a date with Medeama awaits. Shafiu Mumuni gave the Miners an early lead but the club had to rely on Emmanuel Owusu's goal to progress as Abdul Rahman's 23rd-minute effort had levelled the score.
Medeama, meanwhile, beat Samartex 9-8 on penalties to qualify.
The last quarter-final fixture will be between Heart of Lions and Nzema Kotoko. Whereas Lions beat Kotoku Royals 2-0, and Nzema handed Vision FC a 3-0 defeat in Saturday's only game.