Their opponents are likely to provide sterner opposition than Arsenal did on Saturday, with the Italian champions boasting a stunning home record.
Indeed, Lazio are on the only team in all competitions to have won a game at the Allianz Stadium since August 2015 and Spurs know they are in for a serious test on Tuesday.
The hosts will be without a number of key players as Andrea Barzagli, Juan Cuadrado, Douglas Costa and Blaise Mautuidi are all set to miss the visit of the north Londoners.
Paulo Dybala is also a doubt for the game with a hamstring problem.
Spurs are without centre-back Toby Aldeweireld, who has not travelled to Turin.
Tight first leg in Turin
Given their vastly superior European experience and their exceptional home record, Juve are rightly clear favourites.
Spurs, however, have shown themselves to be capable of competing with the continent's best and are very capable of keeping things tight on Tuesday evening.
The hosts will also be desperate to keep a clean sheet and avoid conceding an away goal meaning there is every chance of a low-scoring game on Tuesday evening.
Tips and predictions
Both of these teams boast extremely strong defensive units and there is every chance that one of them will keep a clean sheet.
Furthermore, this selection paid out in four of Juve's six games in the group stages of the tournament.
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