Age Hareide's charges kicked off their campaign with a hard-fought win against Peru last weekend and the Danes can take a significant stride towards a place in the last 16 with a win here.
Denmark are chalked up as 10/11 (1.91) favourites with SportPesa to see off the Socceroos, while the same firm go 12/5 (3.40) that both sides are forced to settle for a share of the spoils at Samara Arena.
Australia have won just one of their nine World Cup finals fixtures against European opposition and SportPesa offer 33/10 (4.30) that Bert van Marwijk's charges breathe fresh life into their campaign with a famous victory.
Andrew Nabbout was chosen to lead the line against France in Australia's opening fixture and could start again here, although Tomi Juric could come into contention for a starting spot.
Andreas Christensen and William Kvist were both withdrawn due to injury against Peru; the former is likely to be fit to feature, but the latter will play no further part in the competition.
Kasper Schmeichel made a string of fine saves to deny Peru in Denmark's opening Group C game and will hope to continue that fine form here.
Eriksen set to shine?
Tottenham star Christian Eriksen is a key protagonist for Hareide's men and could be the difference between whether the country make it out of the group.
Indeed, the midfield maestro has scored 12 goals in his last 14 appearances for his country and SportPesa offer 12/5 (3.40) that Eriksen scores at any time against Australia.
Denmark's defensive discipline was the key to their success against Los Incas in their opening group game and the shot-shy Socceroos may struggle against Hareide's well-drilled side.
The Danes have kept a clean sheet in each of their last five matches and SportPesa's 11/10 (2.10) quote for Schmeichel and his side to chalk up yet another clean sheet could represent good value here.