Marco Asensio, Koke and Nacho have been drafted into the starting XI in what is likely to be merely a technical decision.
Nacho, meanwhile, replaces Real Madrid team-mate Carvajal at right-back, with the latter also having had a sub-par performance against the Atlas Lions.
For Russia's part, the hosts look to be setting up a 3-4-3 against La Furia Roja, which signals the first time they have deviated from their 4-2-3-1 since the start of the tournament.
Aleksandr Samedov and Aleksandr Golovin will provide support for lone strike Artem Dzyuba, though will likely tuck in when Spain have possession to create a congested midfield in order to cut passing lanes.
Spain starting XI: De Gea; Nacho, Pique, Ramos, Alba, Koke, Busquets, Silva, Isco, Asensio, Costa.
Subs: Carvajal, Iniesta, Saul, Rodrigo, Thiago, Vazquez, Odriozola, Kepa, Azpilicueta, Monreal, Aspas, Reina.
Russia starting XI: Akinfeev; Fernandes, Kutepov, Ignashevich, Kudryashov, Zhirkov, Kuzyaev, Zobnin, Samedov, Golovin, Dzyuba.
Subs: Semenov, Cheryshev, Gazinskiy, Dzagoev, Smolov, Lunev, Granat, Aleksey Miranchuk, Anton Miranchuk, Erokhin.