Mancini confirmed Jovetic will be unlikely to play at Stadio Luigi Ferraris on Sunday, and trusts that the Manchester City-loanee will be sensible on international duty as he aims to have him fit to face Juventus on October 18.
"There's no problem, I'm not angry with anyone. It's normal for him to be called up. I don't think he's able to play [against Sampdoria]," Mancini said.
"He's a smart lad. If he's called, he has to go, but he knows what to do. I hope he can return to the pitch to face Juventus."
And Mancini was shocked by the manner of the defeat, as he believes his side played some of their best football of the campaign, especially in the first half.
"It was an absurd defeat. In the first half-hour, despite our disadvantage, we played one of our best games," he said.
"It can't be explained. You accept the result and you move on. We lost an important player in Miranda, that's the main bad point.
"You don't lose the game because you play three or four at the back. It's down to other reasons, like individual mistakes or errors from the referee.
"We've taken 15 points from six games, I don't think we're playing too badly. I'm not worried by what the papers say. I have an idea of football and I won't let it be influenced by external factors."
Mancini expects a tough test against Sampdoria next, with Walter Zenga's side currently five points off the top of the table after netting 12 times in their first six games.
He continued: "Samp are a good team and have three or four quality players in attack who can decide a match. I'm happy to see Walter back in Italian football, he's doing a great job."
Despite their loss last time out, the San Siro giants are still joint-top of Serie A - a position they share with their recent conquerors, Fiorentina.