Aubameyang helped Daniel Cousin's men defeat South Sudan on October 12 but missed the team's trip to South Sudan for the return fixture four days later
The Panthers lock horns with leaders Mali at Stade d'Angondje in a battle for supremacy in Group C on November 12.
And the Gunners star who has scored nine goals in 14 matches across all competitions this season joins Southampton’s Mario Lemina and Cardiff City defender Bruno Manga in the 23-man list.
A win in Libreville will secure Gabon's spot for the continental showpiece billed for Cameroon next year.
Goalkeepers: Didier Ovono Ebang, Yves Stephane Bitseki Moto,
Defenders: Lloyd Palun, Bruno Ecuele Manga, Henri Junior Ndong, Aaron Appindangoy, Johann Serge Obiang, Franck Perrin Obambou, Wilfried Ebane.
Midfielders: Mario Lemina, Andre Biyogo Poko, Lévy Clement Madinda, Medwin Biteghe Medane, Samson Mbingui, Clech Loufilou, Franck Engonga
Forwards: Pierre Emerick Aubameyang, Gaetan Missi Mezu, Axel
Standby: Guélor Kanga, Ulysse Ndong, Johan Didier Lengoualama.