Record-holders Kaizer Chefs will visit NFD side Stellenbosch United. Mamemlodi Sundowns will host minnows Marvien United.
The round's stand-out tie is undoubtedly the KZN derby which will see Golden Arrows and Maritzburg United lock horns.
The other all-PSL showdown will see Highlands Park face Free State Stars.
The first round clashes will be played on the first weekend of March. The dates, venues and kick-off times will be confirmed by the PSL cup committee in due course.
Kwadukuza United v Ajax Cape Town
Cape Town All Stars v Bidvest Wits
Royal Eagles v SuperSport
Stellenbosch United v Chiefs
EC Bees v Pirates
Sundowns v Mariveni United
Polokwane City v African All Stars
Platinum Stars v AmaZulu
Buya Msuthu v Celtic
FC Cape Town v Baroka FC
Chippa United v Witbank Spurs
Jomo Cosmos v United Rovers
Acornbosh Unted v Cape Town City
Days FC v Mbombela United
Highlands Park v Free State Stars
Golden Arrows v Maritzburg United