Diego Simeone's men were superb against the Serie A champions three weeks ago and they will be hoping to finish the job in Turin.
If Los Rojiblancos score on Tuesday, then Max Allegri's side will need to net four times. An unlikely feat but Atletico did concede four goals at Borussia Dortmund in the group stages, and Juve boast the competition's all-time leading scorer in Cristiano Ronaldo.
Juventus have won fifteen of their eighteen home matches in all competitions this term, drawing two and losing just once. The Turin side are offered at 4/6 (1.66) with bet365 to claim another victory but at 3/1 (4.00) to qualify.
Juve have a trio of players missing with Sami Khedira, Juan Cuadrado and Douglas Costa all likely to be out for Tuesday's showdown. Cristiano Ronaldo was an unused substitute on Friday and will return to the starting eleven.
Atletico are without midfielder Thomas Partey, who is suspended, while captain Diego Godin is a major doubt.
If there is a side capable of defending a two-goal lead away from home in Europe it is Atletico Madrid. Indeed, Los Rojiblancos currently boast the best defence in La Liga, as they have done in each of the three previous seasons.
This term in the Champions League they have kept four consecutive clean sheets, although Juve's scoring record is impressive - netting in their last 22 matches in front of their own fans in all competitions.
They will go for goals from the off against Atletico and it is difficult to back against them finding the back of the net. However, they can certainly be contained by an Atletico side that are experts in maintaining an advantage.
Tips and predictions
Juventus are offered at 5/6 (1.83) to score under 1.5 goals and Atletico have restricted their opponents to one goal or less in ten of their last thirteen away games in the Champions League.
Odds correct at the time of writing. Please gamble responsibly.