The 40-year-old will not be offered an extension to a playing contract due to expire this summer and is set to bring the curtain down on a remarkable one-club spell on Sunday.
He will, however, need to be introduced as a substitute if he is to make his 786th outing.
Totti made his debut for Roma in March 1993, having stepped out of the academy ranks at his home-town club.
He has gone on to become the Serie A side’s all-time leading appearance maker and goalscorer, with 307 efforts to his name.
A directorial post is waiting for him at Roma, but no decision has been taken as yet regarding a possible spell elsewhere before hanging up his boots.
Roma may need Totti to offer some inspiration against Genoa, with second place in the Italian top-flight still up for grabs.