Mauricio Pochettino's side will play their home games under the lights of the national stadium for the duration of the 2017-18 season, with Italian champions Juve kicking off proceedings in this weekend's friendly.
With Spurs starting their Premier League season in the North East against newly-promoted Newcastle, Saturday presents Pochettino and his team an opportunity to get used to their temporary surroundings before hosting rivals Chelsea in their Premier League debut at the 90,000-capacity ground.
Juventus are looking to continue their strong pre-season form ahead of the new Serie A campaign, as they prepare to challenge for what would be their seventh consecutive Scudetto.
|Game||Tottenham Hotspur vs Juventus|
|Date||Saturday, August 5|
TV CHANNEL & LIVE STREAM
In the UK, the match will be available to watch live on television on Premier Sports.
|UK TV channel|
In the US, the match will be available to watch live on television on ESPN, and streamed via WatchESPN.
|US TV channel||Online stream|
SQUAD & TEAM NEWS
|Goalkeepers||Hugo Lloris, Michel Vorm|
|Defenders||Danny Rose, Toby Alderweireld, Jan Vertonghen, Kieran Trippier, Kevin Wimmer, Ben Davies, Cameron Carter-Vickers|
|Midfielders||Erik Lamela, Victor Wanyama, Georges-Kévin N'Koudou, Eric Dier, Moussa Sissoko, Mousa Dembélé, Dele Alli, Christian Eriksen, Josh Onomah, Harry Winks|
|Forwards||Son Heung-min, Vincent Janssen, Harry Kane|
Pochettino is likely to name a full-strength Spurs side ahead of the tie, with Kieran Trippier to retain his place at right-back. The 26-year-old has a decisive season ahead of him following Kyle Walker's transfer to Manchester City.
Possible line-up: Lloris; Vertonghen, Dier, Alderweireld; Trippier, Winks, Dembele, Davies; Dele, Eriksen; Kane
|Goalkeepers||Gianluigi Buffon, Carlo Pinsoglio, Wojciech Szczęsny|
|Defenders||Mattia De Sciglio, Giorgio Chiellini, Medhi Benatia, Alex Sandro, Andrea Barzagli, Daniele Rugani, Stephan Lichtsteiner, Dario Del Fabro|
|Midfielders||Miralem Pjanić, Sami Khedira, Juan Cuadrado, Claudio Marchisio, Mario Lemina, Kwadwo Asamoah, Stefano Sturaro, Tomás Rincón, Rolando Mandragora, Rodrigo Bentancur|
|Forwards||Gonzalo Higuaín, Douglas Costa, Marko Pjaca, Mario Mandžukić, Paulo Dybala, Federico Bernardeschi, Moise Kean|
Spurs are set to face a strong Juventus side on Saturday, with Paulo Dybala and Gonzalo Higuain providing the firepower in the forward positions.
Max Allegri is likely to re-shuffle his defence after the club's 1-1 friendly draw with Roma, with Giorgio Chiellini likely to partner Daniele Rugani at centre-back.
Possible line-up: Buffon; De Sciglio, Chiellini, Rugani, Sandro; Khedira, Pjanic; Costa, Dybala, Bernardeschi; Higuain
BETTING & MATCH ODDS
Spurs are 17/10 to defeat their Italian opponents on Saturday, with the Serie A champions placed at 11/8 to win.
Dabblebet are also offering 11/8 for the draw.
Spurs will be looking to get their Wembley monkey off their back before the 2017-18 Premier League campaign begins, as they prepare for their first league campaign away from White Hart Lane.
The north London club's poor record at the national stadium has been well documented, and while the result against the Italian champions on Saturday means little in terms of overall gain, it will provide hope for supporters that the club can in fact settle into their temporary home ahead of the season.
Spurs started their pre-season camp with a 4-2 victory over Paris Saint-Germain in the International Champions Cup, before conceding six in their next two outings.
Pochettino's side lost 3-2 to Roma, before Manchester City beat them 3-0 in their final ICC fixture.
Juventus, who also competed in the ICC pre-season tournament, will use the tie as a final run out before tackling Lazio in the Super Cup final on August 13.
Chiellini, Sami Khedira and Douglas Costa are likely to be recalled to Max Allegri's starting XI having been used as substitutes in the club's 1-1 with Roma last week, while new signing Federico Bernardeschi could be handed his first unofficial start for the Turin side.
Tottenham and Juventus met last summer in an ICC tie which resulted in the Italian side winning 2-1, and while neither side will care too much about the final result at Wembley on Saturday, both managers will be looking for a psychological victory ahead of the new campaign.