Samatta was doubtful for the match following a knee injury sustained when his national team played Burundi in a 2022 Fifa World Cup qualifier.
The 26-year old missed Friday's league game in which his side lost 2-1 to their hosts Sporting Charleroi. The striker has however passed a medical examination and is part of the squad which travelled to Austria for Tuesday's game.
The match will be played at the Wals-Siezenheim stadium, commonly referred to as Red Bull Arena.
Mbwana joined Genk from TP Mazembe of DR Congo in 2016 and has made over 85 appearances for the club, scoring more than 40 goals in the process.