The squad which includes notable names like skipper Asamoah Gyan, Christian Atsu, Thomas Teye Partey and Harrison Afful, is conspicuously without the Ayew brothers, who according to reports, have fallen out with head coach Kwesi Appiah.
The duo missed out the Black Stars' 5-1 victory in the last game against Congo in Brazzaville, after insisting they were not fully fit and needed further checks at their respective clubs.
The 56-year-old has handed a debut call-up to Black Stars B skipper Isaac Twum alongside Vincent Atingah, who both played a huge role in the country's triumph over Nigeria to lift the Wafu Cup.
Also, Kingsley Sarfo has been handed another opportunity after he turned down his maiden call-up in June, as he was hoping to rather represent Sweden, while Daniel Opare has been handed his first call-up in more than three years, following his splendid start to the German Bundesliga this season with Augsburg.
Raphael Dwamena, who saw his deal with Premier League side Brighton & Hove Albion hit a snag after a failed medical, has been handed a major boast to regain his lost confidence and Richmond Boakye Yiadom has maintained his place in the team after his brace against Congo.
Goal understands that the Black Stars will camp in Kenya and Appiah is expected to reduce the list to 23 before they travel to Uganda.
Ghana need a victory to stand a place to qualify to the World Cup, as they sit third in Group E with five points, four points behind Egypt, who host Congo in the other group game.
Defenders : Harrison Afful (Columbus,USA), Daniel Opare (Augsburg, Germany) Agbenyenu Lumor (Portimonese, Portugal), Abass Mohammed (Harrisburg City, USA), Kasim Nuhu (Young Boys, Switzerland), Daniel Amartey (Leicester,England), Jonathan Mensah (Columbus Crew,USA), Nicholas Opoku (Club Africain, Tunisia), Vincent Atingah (Hearts of Oak)
Midfielders: Thomas Partey (Atletico Madrid, Spain), Joseph Attamah (Bakaksehir, Turkey), Christian Atsu (Newcastle United, England), Kingsley Sarfo (Malmo, Sweden) Alfred Duncan (Sassuolo FC, Italy), Ebenezer Ofori (Stuttgart, Germany), Thomas Agyepong (NAC Breda, Holland), Frank Acheampong (Tianjin TEDA, China), Isaac Twum (Inter Allies), Patrick Twumasi (Astana FC, Kazakhtan)