The Citizens secured their third win in four league matches when they defeated Mamelodi Sundowns 2-1 at home last weekend.
Benni McCarthy will look to guide City to victory over Polokwane which would take his side to the second spot - a point behind leaders, Sundowns.
They will face a Polokwane side which is currently enjoying a five-match unbeaten run in the league having defeated Golden Arrows 2-0 at home last weekend.
Rise and Shine are placed sixth on the league standings and they are three points behind third-placed Cape Town City.
A victory for Polokwane over City will see Jozef Vukusic's side move closer to the pace-setters in the race for this season's PSL title.
Cape Town City defeated Polokwane City 2-1 at the Old Peter Mokaba Stadium in the first round league clash earlier this season.
Meanwhile, an unpredictable Bloemfontein Celtic side will host Bidvest Wits in the Free State Stars.
Siwelele recorded their third draw in their last four league matches when they drew 0-0 with Orlando Pirates away last weekend.
Celtic are placed eighth on the league standings and they are only a point ahead of Highlands Park, who are currently placed ninth on the log.
Lehlohonolo Seema will be eager to lead Celtic to victory over Wits which would see his side cement a spot in the top eight.
They will take on a wounded Wits side as the PSL title contenders are winless in their last four games having lost 2-0 to AmaZulu FC away last weekend.
As a result, the Clever Boys are placed fourth on the league standings and they are two points behind second-placed Pirates.
A win over Celtic will take Gavin Hunt's Wits to the second spot if the result between Cape Town City and Polokwane City goes their way.
Celtic secured a 2-1 win over Wits at the Bidvest Stadium in the first round league clash earlier this season.
|Match Number||Fixture||Date||Venue||Kick-off Time||TV channel|
|1||Cape Town City v Polokwane City||15/03||Cape Town Stadium||20h00||N/A|
|2||Bloemfontein Celtic v Bidvest Wits||15/03||Dr Petrus Molemela Stadium||20h00||N/A|