David Alaba and Javi Martinez played a full part in Sunday's training session and could be ready to start at the Santiago Bernabeu on Tuesday, although Jerome Boateng has been ruled out.
"The mood within the team is very, very good," Alaba said. "You can see in training how focused and concentrated we are.
"In the dressing room you can tell how much we want it. We'll throw everything we have at it.
"I'll obviously keep doing all I can to stay on track [to play]."
Left-back Alaba's return will be welcomed by Bayern after his replacement Rafinha was badly exposed in Madrid's 2-1 victory, the Brazilian's loose pass setting up a break for Marco Asensio to hit the winner.
Madrid have injury problems of their own, with defenders Nacho and Dani Carvajal both doubts, meaning Lucas Vazquez may need to fill in at right-back, as he did in the closing stages in Munich.
Isco - who trained on Sunday - is also battling to recover after sustaining a shoulder injury in the first leg, which forced his half-time withdrawal for matchwinner Asensio.