The 26-year-old last featured for Mauricio Pochettino’s men in the round of 16 against Borussia Dortmund after suffering a hamstring problem.
The former Paris Saint-Germain right-back will also not play a part when the Lilywhites host Pep Guardiola’s men at Wembley as he continues with his rehabilitation, with Kieran Trippier expected to start.
TEAM NEWS:— Tottenham Hotspur (@SpursOfficial) April 8, 2019
🔹 @Serge_aurier (hamstring)
🔹 @ericdier (hip flexor)
🔹 @ErikLamela (hamstring)
All three players are undertaking on-field rehabilitation and remain unavailable for selection.#UCL ⚪️ #COYS pic.twitter.com/s7oPToFleL
Aurier has been limited to 16 appearances in all competitions for Spurs this season.