Reus has been out of action since sustaining a partial tear to the anterior cruciate ligament in his right knee during Dortmund's DFB-Pokal final victory over Eintracht Frankfurt last May.
He will feature from the start at Signal Iduna Park in an attacking line-up that also includes Andre Schurrle, Shinji Kagawa, Christian Pulisic and Michy Batshuayi.
Germany international Reus has been plagued by injuries over the past three years but will hope to string together an impressive run of form ahead of the World Cup.
Reus has been plagued by an assortment of leg injuries over the past four years which have limited him for both club and country. He played in just 17 Bundesliga contests last season.
Both Pulisic and Schurrle keep their place despite Reus' return. Schurrle and Pulisic teamed with Reus in behind Chelsea loanee Batshuayi and ahead of Kagawa and Julian Weigl.
Dortmund's starting XI also sees Manuel Akanji handed his first start having made his debut off the bench in last week's 3-2 victory over Cologne.
The club enters play in fourth place in the table with the chance to go up to third depending on results today.