Barring a monumental upset, Diego Simeone's men will surely book their place in the quarter finals and maintain their status as tournament favourites.
Strikers Diego Costa and Kevin Gameiro have not travelled to Russia, while goalkeeper Jan Oblak may be rested.
Lokomotiv's top scorer Jefferson Farfan picked up a muscle injury in the previous round against Nice, but could be in contention for a return.
Atletico to grind out a result in Moscow
There are few teams in Europe better at defending a lead than Diego Simeone's side and with a 3-0 first leg advantage, expect Los Rojiblancos to see out a positive result in Russia.
The visitors will not necessarily be going for goals, but they are more than capable of keep another clean sheet in what should be a low-scoring game.
Tips and predictions
Atletico are priced at 13/10 (2.30) to keep a clean sheet and that looks like an extremely tempting option.
Madrid have kept clean sheets in their away trips to Qarabag and Roma in the Champions League earlier this season, while domestically they have kept clean sheets in eight of their last 11 La Liga matches.
Also worthy of consideration is backing under 1.5 goals in the game at 7/4 (2.75), which should have a good chance of paying out.Atletico Madrid clean sheet at 13/10 (2.30) for a 2pt stake with SportPesa