Gareth Southgate's side have a 100 per cent record in Group A after four games and a win over the second-placed Czechs will see them move six points clear at the top of the table.
The hosts, meanwhile, come into the match just three points behind the group leaders, but they are worriedly looking over their shoulders at Kosovo, who are hot on their heels in the race for qualification.
Despite suffering a heavy defeat to England when the sides met at Wembley in March, Jaroslav Silhavy will have full confidence in his side to peform at home.
|Game||Czech Republic vs England|
|Date||Friday, October 11|
|Time||7:45pm BST / 2:45pm ET|
TV Channel, Live Stream & How To Watch
In the United States (US), the game is not being shown live on TV but it can be streamed live online using ESPN+ or TUDNxtra.
|US TV channel||Online stream|
|N/A||ESPN+ / TUDNxtra|
In the UK, the match will be shown on ITV and it can be streamed live online via the ITV player.
|UK TV channel||Online stream|
Squads & Team News
|Position||Czech Republic squad|
|Goalkeepers||Vaclik, Pavlenka, Kolar|
|Defenders||Kaderabek, Brabec, Celustka, Boril, Coufal, Kudela, Simic|
|Midfielders||Darid, Krejci, Jankto, Soucek, Husbauer, Kopic, Zmrhal, Masopust, Kral, Kalvach|
Czech Republic will be without Trabzonspor defender Filip Novak, as well as Kasimpasa's David Pavelka and Slavia Prague's David Hovorka, who have all been ruled out through injury. Experienced Augsburg defender Marek Suchy is another absentee.
Head coach Silhavy will look to his captain Vladimir Darida and the likes of RB Leipzig striker Patrik Schick to lead his side to glory.
Confirmed Czech Republic XI: Vaclik, Coufal, Celutska, Brabec, Boril, Soucek, Kral, Masopust, Darida, Jankto, Schick.
|Goalkeepers||Pickford, Pope, Henderson|
|Defenders||Trippier, Rose, Keane, Maguire, Alexander-Arnold, Chilwell, Gomez, Mings, Tomori|
|Midfielders||Rice, Barkley, Henderson, Winks, Mount|
|Forwards||Kane, Sterling, Sancho, Rashford, Abraham, Wilson|
In-form Leicester City winger James Maddison withdrew from the squad with an illness, meaning he will have to wait for his Three Lions debut.
Everton midfielder Fabian Delph and Aston Villa goalkeeper Tom Heaton have also been forced to withdraw with injuries, while Manchester United star Jesse Lingard's difficulties at club level have seen him excluded.
Chelsea defender Fikayo Tomori could make his debut as he is on the bench, while Bournemouth's Tyrone Mings misses out through illness.
Meanwhile, Mason Mount makes his first England start, partnering Jordan Henderson and Declan Rice in midfield.
Confirmed England XI: Pickford, Trippier, Keane, Maguire, Rose, Henderson, Rice, Mount, Sterling, Kane, Sancho.
Betting & Match Odds
England, who already registered a 5-0 win over their hosts in March, are 8/13 to win with bet365. Czech Republic are considered a 5/1 bet to come out on top and a draw is available at 16/5.
England will join the likes of Belgium as one of the first teams to ensure their place in next year's European Championship if they manage to beat Czech Republic and the signs are positive.
Southgate's side have been in free-scoring form during this campaign, registering an average of 4.75 goals a match thus far, with their only real wobble coming in last month's 5-3 win over Kosovo.
The front three of Harry Kane, Raheem Sterling and Jadon Sancho have been particularly impressive, scoring 14 of their 19 goals, with pundits suggesting that they are among the most potent trios in the world.
Interestingly, however, Sterling - who bagged a hat-trick against the Czechs last time out - is of the belief that they have not yet reached the level of other world-class trios.
“I think that there are a lot of great front threes in the world,” said Sterling ahead of the game against the Czechs.
"They’re all players that are still getting better, still learning, but at the same time are producing as well, so it’s an exciting front three, but you know, we’ve got to do it in big games, big tournaments.
“That’s when we’ll be judged if we’re a great front three or not.”
The bar has been set high by Southgate and the Three Lions boss will no doubt be expecting his players to deliver qualification early so that he can get a head start on laying the foundations ahead of the tournament.