The Socceroos now preliminary 27-man squad will be cut by four in the aftermath and there's plenty on the line for a number of Socceroos against an opponent that can't be underestimated.
Having beaten China 4-1 in their last outing, the Karel Jarolím-coached side will be eager to put Bert van Marwijk's team to the test and sit just five places lower than the Socceroos in the FIFA world rankings.
|Game||Czech Republic vs Australia|
|Date||Friday June 1|
|Time||13:00 LOCAL / 21:00 AEDT|
TV Channel & Live Stream
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The match will also be telecast live on Foxtel via Fox Sports and picked up on free-to-air via Channel Ten's sister channel One.
|AUS TV channel||Online stream|
|Fox Sports/ One / One HD||Foxtel Now / Foxtel Go|
Squads & Team News
|Goalkeepers||Ryan, Jones, Vukovic|
|Defenders||Behich, Degenek, Jurman, Meredith, Risdon, Sainsbury, Karacic|
|Midfielders||Brilliante, Irvine, Jedinak, Luongo, Milligan, Mooy, Petratos, Rogic, Troisi|
|Forwards||Cahill, Juric, Kruse, Leckie, Nabbout, Rukavytsya, Arzani, Maclaren|
With three players set to be cut from the squad just days after this game, expect every member of this squad to be giving it their all.
Bert van Marwijk should give fringe players a chance to prove their case for a World Cup spot with Fran Karacic and Daniel Arzani both in line for Socceroos debuts.
After giving Brad Jones and Danny Vukovic a half each in the Socceroos last outing against Colombia, Mat Ryan should be restored in goal as he looks to make the spot his own.
In defence, Karacic will likely start at right-back with Josh Risdon then likely given minutes in the second half as they square off for the position.
Having missed both friendlies against Colombia and Norway, Trent Sainsbury and Matt Jurman should be given a chance to settle in the back line.
In the middle of the park, captain Mile Jedinak may well be given a rest after his recent game for Aston Villa allowing Van Marwijk to experiment more freely with the Socceroos midfield.
Jackson Irvine, Dimi Petratos, Andrew Nabbout and Nikita Rukavytsya should all get minutes too as they look justify their place in the final squad.
Up front, there's a chance Tim Cahill could get a start with the veteran striker in desperate need of match minutes after spending most of his club season on the bench.
Meanwhile, Jamie Maclaren will be desperate to impress after being called back into the squad as Tomi Juric battles a knee injury.
Potential Australia starting XI: Ryan; Karacic, Sainsbury, Jurman, Behich; Irvine, Mooy, Arzani, Nabbout, Rogic; Cahill.
|Position||Czech Republic players|
|Goalkeepers||Koubek, Pavlenka, Vaclík|
|Defenders||Bořil, Selassie, Jugas, Kadeřábek, Kalas, Novák, Suchý|
|Midfielders||Barák, Darida, Hušbauer, Jankto, Kopic, Souček, Zmrhal|
|Forwards||Krmenčík, Schick, Vydra|
Betting & Match Odds
Despite being ranked lower than the Socceroos in the world rankings, Czech Republic will enter this friendly as slight favourites at $2.40 with Australia sitting at $2.90 and the draw valued at $3.25 with odds courtesy of Bet365.
With the Socceroos struggling for goals of late and the Czechs known for a strong defence, don't expect too many goals at the NV Arena with odds of less than 2.5 goals being scored going for $1.65.
There's no such thing as a friendly in football and with spots in the Socceroos World Cup squad on the line, expect plenty of desperation from the players out on the pitch.
With 26 set to turn to 23 not long after the match, every minute will count against a Czech Republic side that won't be making the audition an easy one for Australia's players.
Fresh off a 4-1 win over China last time out, the Czechs will be looking to cash in on a Socceroos side still finding their feet under Van Marwijk.
Expect further stumbles too from an Australian side that will be somewhat experimental as the Dutch coach tries to determine which three players are expendable from his current crop.
Arzani and Karacic are likely to be given their first taste of senior national action and will need to defy their youth to book a spot on the plane to Russia.
On the other end of the spectrum is a 38-year-old Cahill, who, though ever-reliable in the national arena, needs match minutes to prove he can still contribute for the Socceroos on the biggest stage.
Czech Republic have won five of their last six matches, while Australia have tasted victory in only three of their last 12.
With a World Cup fast approaching and a tough group to battle, the Socceroos need to rediscover that winning feeling quickly if they are to defy the odds in Russia.
Unfortunately, a victory may be one step too far for an Australian outfit that will likely be chopped and changed throughout the game giving the Czechs a distinct advantage.
These two sides have met just once before, back in 2000 when Czech Republic claimed a 3-1 win.