Video Assistant Referee (VAR) technology will be used during Monday's FA Cup third round tie between Brighton and Crystal Palace
The technology, which was used at the 2016 Club World Cup, has also been used in Germany, Italy and at the Confederations Cup.
VAR can be used in four separate situations, termed as "match-changing": goals, penalties, straight red cards and cases of mistaken identity.
England's friendly against Germany in November was the first time VAR had been available in this country, though the referee did not call upon it, while it will also be used in the Carabao Cup semi-final encounters between Chelsea and Arsenal, as well as the final.
Two teams of referees will monitor footage from London, and a screen will be set up at the side of the field of play, for the referee's use.
The referee can ask for VAR to be employed, or an official can be advised to review a decision using the tech.
Former referee Mike Riley, manager of the Professional Game Match Officials Board, believes that there will need to be an adjustment period for most refs, and insists VAR is not "100% perfect".
"It's going to take us time," he told the BBC. "You're actually asking a generation of referees to relearn or learn new processes and skills.
"It will never be 100% because it's so subjective on certain things and we're asking the clear and obvious question.
"We don't want errors. If through this we make that 4% [of incorrect decisions] 2%, we've benefited the game."