The Liverpool striker continues to struggle with a thigh injury and will not travel to Kiev, leaving England manager Roy Hodgson with limited options on Tuesday night
Sturridge – who has scored five goals in four matches for Liverpool this season – was ruled out of Friday's 4-0 win over Moldova at Wembley due to the problem.
And the striker will not be risked by England manager Roy Hodgson in Kiev on Tuesday, despite the crucial nature of the fixture, which could play a big role in deciding who qualifies automatically from Group H for World Cup 2014.
A statement from the Football Association read: "The FA can confirm that Daniel Sturridge will not return to the England squad ahead of the World Cup qualifier with Ukraine on Tuesday.
"Club England medical staff have been in constant contact with their counterparts at Liverpool since he returned there on Thursday, his injury has not progressed as had been hoped and it is now clear that Sturridge will not be fit for the match in Kiev on Tuesday evening.
"No additions will be made to Roy Hodgson's squad of 23 players who travel to Ukraine on Sunday evening."
Manchester City midfielder James Milner is expected to fill Sturridge's role from the start in Kiev.
Sturridge has scored in each of Liverpool's three Premier League matches, helping the Anfield outfit to the top of the table.
The latest news surrounding his injury could leave him a doubt for Liverpool's trip to Wales to face Swansea in their next match on September 16.