The 34-year-old last just 15 minutes of the 2-1 defeat on Tuesday night, with Roberto Di Matteo confirming a recurrence of a calf injury, and will now have a scan ahead of Sunday
The 34-year-old lasted only 15 minutes of the 2-1 defeat before succumbing to a recurring calf problem that ruled the England international out of the San Marino and Poland games earlier in October.
Boss Roberto Di Matteo will now be sweating on the fitness of his vice-captain for the clash with the Red Devils as club captain John Terry serves the second of his four-game domestic ban.
"It's basically a recurrence of an old injury,” Di Matteo told reporters.
"He was training with us fine and there were no alarm bells before the game. He was in good condition to play."
Di Matteo also admitted that the loss of Lampard has had a detrimental effect on his pre-match preparations as he fears a demoralised squad after Chelsea slumped to defeat in Donesk to leave the hopes of the holders qualifying for the knockout stages in the balance.
He added: "It's a team that has always shown the character after a defeat and I don't think the previous years should affect the way we are playing this season or where we are this year.