Pair did not speak at the Emirates for over a year after a dispute on the French team bus, with the young midfielder ignoring the centre-back when the sides met earlier this season
The former team-mates did not speak for a year when they played together at the Emirates, and the 23-year-old is refusing to let the grudge drop.
The bitterness between the pair originated from a dispute on a team bus with the French squad in 2008, which led to the defender criticising his young compatriot in an autobiography.
Nasri refused to shake hands with Gallas when they lined up against each other for the first time in November at the Emirates, in a game that Spurs went on to win 3-2.
Nasri said, according to The Sun: "Against Tottenham I refused to shake Gallas's hand. He said to the press 'it's a pity for him'.
"Next time I will not shake his hand either. I'm generous with my family and friends but I am spiteful as well. Defenders who give me kicks know that very well."
The game at White Hart Lane gives Arsenal the chance to bounce back from their disappointing draw to Liverpool on Sunday and an opportunity to cut the seven-point gap to leaders Manchester United.
Boss Arsene Wenger believes his players must be fearless when they take on their cross-town rivals.
He told reporters: "There is a lot at stake. We have not to go with any fear and wait for Spurs to play. We will try to play our game."