With the two nations recording victories over England but falling to defeat against Group D's surprise package Costa Rica, the winner of the clash will join the north American nation in the last-16.
“Now a huge game awaits us,” Cavani said in an interview with Il Mattino. “Certainly it's a difficult one against Italy. We must prepare it as best we can and put in a special performance.
“It is going to be like a final.”
Cavani also took a moment to praise the Uruguay supporters, who have travelled to Brazil in their thousands to support their team.
“Our fans have been fantastic so far in the tournament.” Cavani said, “They remind me of Napoli supporters.”
The Paris Saint-Germain star was a key figure for Uruguay as they beat England in Sao Paulo on Thursday June 19, supplying the cross for Luis Suarez's opener in the first half.
Uruguay captain Diego Lugano missed the victory over England through injury, and it is unclear whether he will be fit for the final group game.