The Manchester United striker kicked out at Jordan Mutch during Sunday's 2-2 draw with Cardiff, and the Sky Sports pundit was vocal in his opinion that a booking was not sufficient
The England star was only booked for the seventh-minute altercation with the midfielder before opening the scoring soon after, with both David Moyes and Malky Mackay deeming the booking as sufficient.
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"I don’t know what Wayne is thinking of," he said. "He’s got an exploding head, we know that. That’s just borne out frustration.
"He should be off, there’s no argument. Why the ref has given him a yellow card is beyond me. He’s got a great view of it . If he’s booked him, he’s seen something. If he sees that, it’s a sending off."
Rooney responded on twitter by taking Sky Sports commentator Martin Tyler and Souness, who he nicknamed "Sourness", to task and questioned why the pair largely igrnored Gary Medel's slap on Marouane Fellaini.
"Just watched game back," Rooney wrote. "I agree mine was bad tackle and ref dealt with it. And Martin Tyler didn’t shut up about it all game.
"Then someone slaps someone in face and he doesn’t speak a word about it. I like Martin but that’s poor from him.
"Sourness the rules are u kick out u should be off. Souness Medel gives left hook he’s done his job. Haha brilliant. Sourness was sarcastic for everyone biting."