Luis Enrique has named his squad to play Granada in La Liga, with the Argentine defender missing out but Sergi Roberto returning
Midfielder Roberto has recovered from the thigh injury which kept him out of Wednesday's Copa del Rey win over Espanyol.
Arda Turan and Aleix Vidal are included in a La Liga squad for the first time after making their club debuts against Espanyol.
Mascherano is joined by Marc Bartra, Douglas, Jordi Masip and Munir El Haddadi in missing Luis Enrique's 18-man party.
Barcelona matchday squad: Bravo, Ter Stegen, Vidal, Alves, Pique, Vermaelen, Mathieu, Adriano, Alba, Busquets, Rakitic, Sergi Roberto, Iniesta, Turan, Messi, Suarez, Sandro, Neymar.