The Manchester United man will miss his country's last match of the group stage due to injury, meaning his Copa America could well be over
The Manchester United winger sustained the knock in his side's scoreless draw with Paraguay and was forced to watch the match against Venezuela from the stands as a result of the problem.
"Valencia still has a lot of pain in his left ankle," team physiotherapist Tony Ocampo stated, according to Eurosport.
The 26-year-old suffered severe bruising in the same place where he underwent surgery last year after breaking his ankle in a Champions League match versus Rangers.
Ecuador currently sit fourth in Group B, and need three points against Brazil on July 14 in order to qualify for the knockout stage.