The France-based playmaker replaces El Kun at the last minute, and is one of several new faces for an Albiceleste team looking to bounce back from disappointment in Venezuela
The former Huracan and Palermo man is a late replacement for Sergio Aguero. The Manchester City player was set to start on the left of the Albiceleste's attacking trident, but suffered a muscle strain in training on Thursday and relinquished his place to 'El Flaco'.
Aside from Pastore, there are several other changes for Alejandro Sabella's team. Clemente Rodriguez returns to international football for the first time since the 2010 World Cup, deputising for Marcos Rojo at left back. There is also a place for Ricardo Alvarez in midfield, as well as the returning Fernando Gago after the former Real Madrid man missed the first two qualifiers.
Bolivia meanwhile have decided not to rest star striker Marcelo Martins, after it was rumoured that the Shakhtar Donetsk striker would be rested until Tuesday's clash with Venezuela. Suspended captain Ronald Raldes is another absence for the Verde, and goalkeeper Carlos Arias is handed the armband.
Argentina: Sergio Romero; Pablo Zabaleta, Martín Demichelis, Nicolas Burdisso, Clemente Rodriguez; Fernando Gago, Javier Mascherano, Ricardo Alvarez; Lionel Messi, Gonzalo Higuain, Javier Pastore.
Bolivia: Carlos Arias; Cristian Vargas, Luis Mendez, Ronald Rivero, Luis Gutierrez; Edivaldo Rojas, Jaime Robles, Walter Flores, Rudy Cardozo; Pablo Escobar; Martins.