The German coach has called on his players to ensure the Real Madrid star has a difficult day when they meet Portugal in Manaus
The United States open their campaign in Brazil against Ghana in Natal on Monday before meeting Portugal in Manaus.
And Klinsmann says nullifying Ronaldo will be vital to their chances of earning a favourable result.
"Our job is to stop Ronaldo in beautiful Manaus," he said.
"We'll think of ways to make it really miserable for him"
The German has played down USA’s chances in Brazil, insisting that football is still growing in the country and stressed the importance of American players joining Champions League clubs in order to further their development.
"Our goal is to get as many players as possible playing in the Champions League," he said.
"On the club level that is the creme de la creme, it is where you want to be, to have that confidence and experience of playing among the best players in the world.
"I think we have very special players on our team that can play there, players that can make it to that level. Right now the statistics prove we are not there yet."
USA end their group campaign against Germany in Recife on June 26.