Eddie Johnson is the most notable omission from the 30-man party which will be reduced to 23 on June 2, with Bayern's Julian Green earning a place
Premier League goalkeeping duo Tim Howard and Brad Guzan are also included, along with Stoke's Geoff Cameron, Julian Green of Bayern Munich and former Roma man Michael Bradely, now of Toronto.
The most notable omission is D.C. United forward Eddie Johnson, who was a regular during the qualifying campaign but saw a dip in form and the emergence of Green end his World Cup dream. Bolton's Tim Ream and Puebla's Michael Orozco were also cited by Klinsmann as near-misses.
Tijuana midfeidler Joe Corona also earned an eyebrow-raising recall after playing only four minutes of the final round of World Cup qualifying, though he did play a role in the 2013 Gold Cup title.
The squad will report to Palo Alto, California, where it will train at Stanford University before the final squad is confirmed June 2.
The full squad:
Goalkeepers: Brad Guzan (Aston Villa), Tim Howard (Everton), Nick Rimando (Real Salt Lake)
Defenders: DaMarcus Beasley (Puebla), Matt Besler (Sporting Kansas City), John Brooks (Hertha Berlin), Geoff Cameron (Stoke City), Timmy Chandler (Nürnberg), Brad Evans (Seattle Sounders), Omar Gonzalez (LA Galaxy), Clarence Goodson (San Jose Earthquakes), Fabian Johnson (Hoffenheim), Michael Parkhurst (Columbus Crew), DeAndre Yedlin (Seattle Sounders)
Midfielders: Kyle Beckerman (Real Salt Lake), Alejandro Bedoya (Nantes), Michael Bradley (Toronto FC), Joe Corona (Club Tijuana), Brad Davis (Houston Dynamo), Mix Diskerud (Rosenborg), Maurice Edu (Philadelphia Union), Julian Green (Bayern Munich), Jermaine Jones (Besiktas), Graham Zusi (Sporting Kansas City)
Forwards: Jozy Altidore (Sunderland), Terrence Boyd (Rapid Vienna), Clint Dempsey (Seattle Sounders), Landon Donovan (LA Galaxy), Aron Johannsson (AZ Alkmaar), Chris Wondolowski (San Jose Earthquakes)